Direct Visualization of Lentivirus Formulations
Cryo-TEM or negative stain TEM imaging and analysis can provide valuable insights and assessment of CQA’s, and inform batch-to-batch variability, scale-up consistency, and process development changes in lentivirus formulations.
Quantitative & qualitative analysis (for R&D purposes or using validated methods) provide a wealth of answers using a single cryo-TEM dataset, only requiring ~50μl of sample.
Cryo-TEM Characterization of Lentiviral Vectors
Cryo-TEM imaging can be used for analysis of lentivirus characteristics, including:
- Morphology (size, shape)
- Particle uniformity
- Particle integrity
- Maturation state
- Surface protein distribution
- Presence of impurities
Cryo-electron tomography provides 3D volumes to better distinguish surface protein distribution and internal particle features.

Other Viral & VLP Formulations we can Image with Cryo or NS TEM

Frequently Asked Questions
How many particles do you count, or images do you analyze per sample or grid?
