Direct Visualization of Lentivirus Formulations

Cryo-TEM or negative stain TEM imaging and analysis can provide valuable insights and assessment of CQA’s, and inform batch-to-batch variability, scale-up consistency, and process development changes in lentivirus formulations.

Quantitative & qualitative analysis (for R&D purposes or using validated methods) provide a wealth of answers using a single cryo-TEM dataset, only requiring ~50μl of sample.  

Cryo-TEM Characterization of Lentiviral Vectors

Cryo-TEM imaging can be used for analysis of lentivirus characteristics, including:  

  • Morphology (size, shape)  
  • Particle uniformity  
  • Particle integrity
  • Maturation state
  • Surface protein distribution  
  • Presence of impurities

Cryo-electron tomography provides 3D volumes to better distinguish surface protein distribution and internal particle features.  

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Comprehensive Characterization of Lentivirus by Cryo or NS TEM

Frequently Asked Questions

microscopic black and white image of lentivirus characterization services with cryo-TEM | nanoimaging services

How many particles do you count, or images do you analyze per sample or grid?

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