Let Our Cryo-EM
Experts Guide You

NanoImaging Services harnesses the power of cryo-EM to help pharma and biotech companies discover and develop novel therapeutics faster than ever before.

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Harness the Power of Cryo-EM with our Three Core Services

SPA Trimer
AAV Structure
Protein Structure
NIS Protein Structure

NIS In Action

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Optimizing a Multi-Component Intranasal Entamoeba Histolytica Vaccine Formulation Using a Design of Experiments Strategy

“Cryo-transmission electron microscopy was performed by NanoImaging Services, Inc…. Formulation morphology of the three lead candidate adjuvant formulations was assessed by cryo-transmission electron microscopy and compared to the morphology of a new batch of the proof-of-concept formulation manufactured at the same time as the lead candidate compositions.”

Division of Infectious Diseases and International Health,
Department of Medicine, University of Virginia Charlottesville, United States
View Case Study
Optimizing a Multi-Component Intranasal Entamoeba Histolytica Vaccine Formulation Using a Design of Experiments Strategy

Structural Arrangement of the VH and VL Domains in the COBRA™ T-cell Engaging Single-Chain Diabody

“We analyzed the structure of an epidermal growth factor receptor (EGFR) COBRA in solution using negative stain electron microscopy (EM) and small-angle X-ray scattering (SAXS)...To understand the structure of EGFR COBRA, we applied negative stain EM followed by single particle analysis that allowed us to visualize domains in this molecule at low resolution.

…[Grids of EGFR COBRA were prepared and] imaging was done by NanoImaging Services (San Diego, CA).”

Senior Scientist at Maverick Therapeutics
"Maverick Therapeutics, Inc., a wholly owned subsidiary of Takeda Pharmaceutical Company Limited, Brisbane, CA, USA"
View Case Study
Structural Arrangement of the VH and VL Domains in the COBRA™ T-cell Engaging Single-Chain Diabody

Cryo-EM in Mechanism of Action Studies: Epitope Mapping of Monoclonal Antibodies Copy

“...we report the cryo-electron microscopy structures of potent and inhibitory anti-hArg antibodies bound to hArg1 which form distinct macromolecular complexes that are greater than 650 kDa…With local resolutions of 3.5 Å or better we unambiguously mapped epitopes and paratopes for all five antibodies and determined that the antibodies act through orthosteric and allosteric mechanisms. ….Grids were prepared and data were collected at NanoImaging Services (San Diego, CA).”

Department of Discovery Chemistry
Merck & Co., Inc., Boston, MA, USA
View Case Study
Cryo-EM in Mechanism of Action Studies: Epitope Mapping of Monoclonal Antibodies Copy

Absolute Configuration Determination Of Pharmaceutical Crystalline Powders By MicroED Via Chiral Salt Formation

“With moderate sample preparation, rapid data processing, and most importantly, unambiguous result interpretation, this [MicroED] approach can serve as a powerful tool in a pharmaceutical scientist's toolbox to determine the absolute configuration of a new API early in drug development, to ensure the quality of drug products, and ultimately, to improve the quality of life of patients.”

Department of Discovery Chemistry
Small Molecule Drug Product Development, Biogen, 115 Broadway, Cambridge, MA 02142, USA
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Absolute Configuration Determination Of Pharmaceutical Crystalline Powders By MicroED Via Chiral Salt Formation

Sugar Phosphate Activation of the Stress Sensor eIF2B

“In this work, we conduct two orthogonal unbiased screens for potential metabolite ligands of eIF2B, identifying sugar phosphates as eIF2Bα binders and enzymatic activators. Using cryo-EM and X-ray crystallography, we elucidate structures of sugar phosphates bound to eIF2B and demonstrate that they occupy the ancestral catalytic pocket within the eIF2Bα subunit…

We thank…NanoImaging Services for assistance with cryo-EM data collection and processing.”

Calico Life Sciences LLC, South San Francisco, CA, USA
View Case Study
Sugar Phosphate Activation of the Stress Sensor eIF2B
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NIS Achievements

150

+

3D structures determined by cryo-EM

76

%

of protein structures solved at 3.5 Å or better resolution

80

%

phasing success rate in MicroED

880

+

Virus, Vaccine and VLP projects completed

50

+

Structures solved by MicroED; Largest structure: 733 Da, Z’=4

200

+

AAV samples imaged; 3D structure of AAV solved to 1.8 Å

2,500

+

Liposomes/LNPs imaged

NIS Molecule

Upcoming Events

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News & Events

COMPPÅ Symposium on Membrane Protein Production and Analysis

Date
June 22, 2022
June 24, 2022
Location
New York, NY
Highlights

25th International Conference on the Chemistry of the Organic Solid State

Date
July 3, 2022
July 8, 2022
Location
Ohrid, Macedonia
Highlights

Jessica Bruhn presenting at Session 1: Crystals in nature and medicine Wednesday, July 6 at 9:40am.

American Crystallographic Association 2022

Date
July 29, 2022
August 1, 2022
Location
Portland, OR
Highlights

Jessica Bruhn presenting at In the Rapidly Advancing Field of MicroED, What Should be Done About Past Data? on Sunday, 7/31 at 3:50pm and Evaluating the Quality of MicroED Structures on Sunday, 7/31 at 7:30pm.

Jessica Bruhn co-hosting Workshop 8: Microcrystal Electron Diffraction (MicroED) with Tamir Gonen on Friday, 7/29 at 8am. Learn more.

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