Direct determination of particle morphology is a critical aspect of nanoparticle characterization. Typical analyses include particle size distribution, shape, and overall appearance of particles in a sample. Of particular importance is lamellarity analysis. Lamellarity is the number of lipid bilayers in a liposome or lipid nanoparticle (LNP). Cryo-TEM can also visualize things like bleb formations in your lipid nanoparticle (LNP). Lamellarity directly affects liposome properties and behavior for example determining encapsulation efficiency and influencing drug release. Therefore, proper characterization of nanoparticle morphology and lamellarity is crucial to understand the potential impact on bioactivity.
Frequently Asked Questions
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Can I see an example report showing your morphology & lamellarity analysis?
Yes! Please contact us to get an example report showing services including lipid nanoparticle (LNP) cryo-TEM imaging at three magnifications along with our Qualitative Assessment, Particle Size Distribution, and Fraction Counting Analysis of morphology & lamellarity.
How many particles do you count, or images do you analyze per sample or grid?
We collect ~60 images from different sections of each grid, usually including different ice thicknesses, and analyze the agreed upon number of particles (between 250 to over 1,000).