Liposomes

Per-Particle Imaging of Liposomes

Cryo-TEM imaging & automated analysis tools can provide insights into liposomal formulations in their near native state, with just ~50μl of sample.

Validated methods & data inform batch-to-batch, scale-up, and process development change comparisons.

Analyze Multiple Liposome CQA’s in One Dataset

Assess many characteristics of liposomes with one cryo-TEM study:

Particle morphology
Lamellarity, including bilayer thickness
Uniformity
Particle size distribution
Estimations of PEG layer thickness
Cargo encapsulation
3D volumes with cryo-electron tomography

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Lipid Nanoformulations We Can Image With Cryo or NS TEM

black and white microscopic image of of lipid nanoparticle nanoparticle LNP characterization | nanoimaging services

Lipid Nanoparticles (LNPs), with mRNA, RNA, DNA payloads
Lipid Nanoparticles (LNPs), with mRNA, RNA, DNA payloads

Services Related to Liposomes

Characterize individual liposomes with cryo-TEM imaging

Liposomes are a leading drug delivery vehicle due to their versatility and ability to provide protection to encapsulated drugs, which enhances stability and prevents degradation.

Liposomes consist of one or more lipid bilayers enclosing an aqueous core, and can be used to encapsulate and deliver a wide variety of both hydrophilic and hydrophobic drugs.

Cryo-TEM imaging studies can visualize individual liposome particles, and reveal particle morphology, lamellarity including bilayer thickness, uniformity, particle size distribution, estimations of PEG layer thickness, and cargo encapsulation. These values can be used to inform batch-to-batch, scale-up, and process development change comparisons.

Cryo-TEM can show you if each individual liposome is unilamellar, multilamellar, or multivesicular. Cryo-TEM imaging studies can also assist in determining PEG layer thickness. For a detailed 3D view of each particle, cryo-electron tomography can be performed.