Analyses

Automated Fraction Counting

Scientific rendering of 3D protein structure, structural biology | Nano Imaging
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Protein and lipid-based nanoparticles have been widely used in clinical applications, such as drug and gene delivery and vaccine development. Their unique physical and chemical properties at the nanoscale level have paved the way for various breakthroughs in medicine and healthcare. Characterizing the homogeneity of nanoparticles is crucial for ensuring their safety, efficacy, and performance in clinical settings.

Cryo-TEM imaging is a powerful technique used to directly visualize nanoparticles, such as liposomes and lipid nanoparticles (LNPs), viruses and virus like particles (VLPs), in their near-native state. This technique can evaluate the size, morphology, internal structure, and surface features of the nanoparticles. Therefore, cryo-TEM imaging is immensely valuable in categorizing nanoparticles based on specific properties of interest.

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Types Nanoparticles We've Imaged and Analyzed

  • Extracellular Vesicles & Exosomes
  • Extracellular Vesicles & Exosomes
  • Iron Nanoparticles
  • Iron Nanoparticles
  • Lipid Nanoparticles (LNPs), with mRNA, RNA, DNA payloads
  • Lipid Nanoparticles (LNPs), with mRNA, RNA, DNA payloads
  • Liposomes
  • Liposomes
  • Micelles
  • Micelles
  • Nanotubes
  • Nanotubes
  • Polymeric nanoparticles
  • Polymeric nanoparticles
  • Protein based nanoparticles
    • Albumin NPs (Abraxane)
    • SARS-CoV-2 subunit vaccine
  • Protein based nanoparticles
    • Albumin NPs (Abraxane)
    • SARS-CoV-2 subunit vaccine
  • Virus-Like Particles (VLPs)
    • Associated Adeno-Virus (AAV) Characterization
    • Hepatitis B Virus (HBV ) Characterization
    • Human Papillomavirus (HPV) Characterization
    • Lentivirus Characterization
  • Virus-Like Particles (VLPs)
    • Associated Adeno-Virus (AAV) Characterization
    • Hepatitis B Virus (HBV ) Characterization
    • Human Papillomavirus (HPV) Characterization
    • Lentivirus Characterization
  • Viruses, attenuated and recombinant (BSL-2 & below)
    • Adenovirus (AV) Characterization
  • Viruses, attenuated and recombinant (BSL-2 & below)
    • Adenovirus (AV) Characterization

Frequently Asked Questions

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Can I see an example report for your fraction counting analysis?

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Yes! Please contact us to get an example report showing services including lipid nanoparticle (LNP) cryo-TEM imaging at three magnifications along with our Qualitative Assessment, Particle Size Distribution, and Fraction Counting Analysis or an example report showing cryo-TEM imaging of adeno-associated virus (AAV) at two magnifications and our automated fraction counting analysis showing the full, empty, and partial capsid ratios.

How many particles do you count, or images do you analyze per sample or grid?

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We collect ~60 images from different sections of each grid, usually including different ice thicknesses, and analyze the agreed upon number of particles (between 250 to over 1,000).

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