Analyses

Negative Stain Screening

NIS Protein Structure
NIS Molecule
NIS Protein Structure

Negative staining is a sample analysis method which reveals particles’ gross structural details using a transmission electron microscope. It is an excellent method for providing a “quick-look” at your sample to evaluate its suitability for further analysis.

Our scientists have many years of experience using negative stain TEM methods.

We also offer optimized negative staining for more detailed assessments of sample behavior. When combined with 2D class averaging, and our SPA workflow, this provides insight into protein domain structure, antibody flexibility and sample integrity and morphology.

Samples We've Imaged

Antibody-antigen complexes
GPCRs
Ion channels
Membrane proteins
Multi-domain proteins
Multi-component complexes
Protein degraders
Protein-nucleic acid complexes
Proteins - with and without ligands
Bacteriophages
Viruses

Samples We've Characterized

Exosomes
Extracellular Vesicles
Iron nanoparticles
Lipid Nanoparticles (LNPs), with mRNA, RNA, DNA payloads
Liposomes
Micelles
Nanotubes
Polymeric nanoparticles
Protein (subunit) based nanoparticles
Virus-Like Particles (VLPs)
Viruses, attenuated and recombinant (BSL-2 & below)
NIS Mountain Hero
NIS Mountain Hero