Analyses

Negative Stain Screening

Scientific rendering of 3D protein structure, structural biology | Nano Imaging
NIS Molecule
NIS Protein Structure

Negative staining is a sample analysis method which reveals particles’ gross structural details using a transmission electron microscope. It is an excellent method for providing a “quick-look” at your sample to evaluate its suitability for further analysis.

Our scientists have many years of experience using negative stain TEM methods.

We also offer optimized negative staining for more detailed assessments of sample behavior. When combined with 2D class averaging, and our single particle analysis (SPA) workflow, this provides insight into protein domain structure, antibody flexibility and sample integrity and morphology.

Types of Proteins We've Imaged

  • Antibody-antigen complexes
  • Antibody-antigen complexes
  • GPCRs
  • GPCRs
  • Ion channels
  • Ion channels
  • Membrane proteins
  • Membrane proteins
  • Multi-component complexes
    • PROTACs
    • Molecular Glues
  • Multi-component complexes
    • PROTACs
    • Molecular Glues
  • Protein degraders
  • Protein degraders
  • Protein-nucleic acid complexes
  • Protein-nucleic acid complexes
  • Proteins - with and without ligands
  • Proteins - with and without ligands
  • Viruses
  • Viruses

Samples We've Characterized

  • Exosomes
  • Exosomes
  • Extracellular Vesicles
  • Extracellular Vesicles
  • Iron nanoparticles
  • Iron nanoparticles
  • Lipid Nanoparticles (LNPs), with mRNA, RNA, DNA payloads
  • Lipid Nanoparticles (LNPs), with mRNA, RNA, DNA payloads
  • Liposomes
  • Liposomes
  • Micelles
  • Micelles
  • Nanotubes
  • Nanotubes
  • Polymeric nanoparticles
  • Polymeric nanoparticles
  • Protein (subunit) based nanoparticles
    • Albumin NPs (Abraxane)
    • SARS-CoV-2 subunit vaccine
  • Protein (subunit) based nanoparticles
    • Albumin NPs (Abraxane)
    • SARS-CoV-2 subunit vaccine
  • Virus-Like Particles (VLPs)
    • Associated Adeno-Virus (AAV) Characterization
    • Hepatitis B Virus (HBV ) Characterization
    • Human Papillomavirus (HPV) Characterization
    • Lentivirus Characterization
  • Virus-Like Particles (VLPs)
    • Associated Adeno-Virus (AAV) Characterization
    • Hepatitis B Virus (HBV ) Characterization
    • Human Papillomavirus (HPV) Characterization
    • Lentivirus Characterization
  • Viruses, attenuated and recombinant (BSL-2 & below)
  • Viruses, attenuated and recombinant (BSL-2 & below)
NIS Mountain Hero
NIS Mountain Hero