Cryo-electron Tomography (CryoET)
A 3D volume of a sample can be reconstructed from a series of 2D projection images acquired by incrementally tilting the sample over a range of angles and acquiring a different image for each tilt. The tilted set of projection images is then mathematically combined to reconstruct a 3D volume of the sample, essentially using tomographic methods (analogous to those used for CT scanning and other medical imaging procedures).
The 3D volume can be examined from different angles and individual slices in different orientations can be extracted to provide a more complete understanding of the particle morphology and resolve any ambiguities of the 2D projection images. This provides an understanding of the shape of particles in 3D relative to their 2D projection images, the distribution of proteins on the surface of drug delivery vehicles, how encapsulated drugs are arranged inside the drug delivery vehicle, particle lamellarity, and particle interactions.
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