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Characterize Extracellular Vesicles & Exosomes with Cryo-TEM Direct Imaging

EVs/exosomes can range in size from 50 nanometers to hundreds of nanometers, depending on their type, source, and cargo. They can also vary in structure, internal cargo, and surface decoration. Unlike any other technique, cryo-TEM allows for direct visualization of each of these attributes. Size distribution analysis and particle classification studies can be used to compare lots and study stability. Immunogold labeling can be performed to confirm the presence of proteins on the surface of EVs/exosomes.

Vesi-Ref CD63 GFP EV standard (Samples provided courtesy of Vesiculab)

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B&W microscopic image of extracellular vesicle and exosome characterization with cryo-TEM | nanoparticle characterization | Nanoimaging Services

How many particles do you count, or images do you analyze per sample or grid?

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