3D Reconstruction of Structures

Understanding the structure of molecules, such as protein structure, is crucial in determining their function. However, obtaining accurate 3D structures from microscopy images can be a challenging and resource intensive process. Electron microscopy, particularly cryo-EM, has gained a reputation for providing images suitable for building 3D reconstructions. In the right hands, these can be used to create highly informative 3D models.

For 3D reconstructions of a heterogeneous sample, such as a liposome or lipid nanoparticle (LNP), cryo-electron tomography is available.