Understanding the structure of molecules such as proteins is crucial in determining their function. However, obtaining accurate 3D structures from microscopy images can be a challenging and resource intensive process. Electron Microscopy, particularly cryo-EM, has gained a reputation for providing images suitable for building 3D reconstructions. In the right hands, these can be used to create highly informative 3D models.
Our experts are skilled in generating high resolution 3D models of challenging molecules, such as membrane proteins, ion channel receptors, GPCRs, antibodies, and emerging viruses.
We cryogenically capture multiple configurations of proteins in a thin layer of vitrified ice, then use the microscope to reveal their shape. We take thousands of images of the molecules from multiple angles, average similar projections to create 2D classes and combine these to construct a 3D representation of the structure.
Our advanced data processing capabilities enable us to identify proteins that are in different stages of a biological process, helping unveil how a structure interacts with its environment, including changes to its conformation and function. We have addressed the challenges of high resolution cryo-EM with the computational infrastructure, including processing power and data storage, required to solve 3D structures, so you don’t have to.
We’ve collected data to support more than 300 studies, solving over 150 structures, of which more than 76% were determined at resolutions of 3.5 Å or better.