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NanoImaging Services harnesses the power of cryo-EM to help pharma and biotech companies discover and develop novel therapeutics faster than ever before.
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Case Studies | NIS In Action
Janssen Research Uses an Integrative Structural Biology Approach to Generate Five Distinct Structures
A team from Janssen Research used an integrative structural biology approach to study human malic enzyme 3 (ME3), a potential therapeutic target for pancreatic cancer.
Anellovirus Structure Reveals a Mechanism for Immune Evasion
Cryo-EM data were collected at NanoImaging Services under the leadership of Dr. Giovanna Scapin and Dr. Phat Dip. Baculovirus was generated by Dr. Peter Pushko at Medigen.
Optimizing a Multi-Component Intranasal Entamoeba Histolytica Vaccine Formulation Using a Design of Experiments Strategy
“Cryo-transmission electron microscopy was performed by NanoImaging Services, Inc…. Formulation morphology of the three lead candidate adjuvant formulations was assessed by cryo-transmission electron microscopy and compared to the morphology of a new batch of the proof-of-concept formulation manufactured at the same time as the lead candidate compositions.”
Structural Arrangement of VH and VL Domains in COBRA™ T-cell Engaging Single-Chain Diabody
“We analyzed the structure of an epidermal growth factor receptor (EGFR) COBRA in solution using negative stain electron microscopy (EM) and small-angle X-ray scattering (SAXS)...To understand the structure of EGFR COBRA, we applied negative stain EM followed by single particle analysis that allowed us to visualize domains in this molecule at low resolution.
…[Grids of EGFR COBRA were prepared and] imaging was done by NanoImaging Services (San Diego, CA).”
Sugar Phosphate Activation of the Stress Sensor eIF2B
“In this work, we conduct two orthogonal unbiased screens for potential metabolite ligands of eIF2B, identifying sugar phosphates as eIF2Bα binders and enzymatic activators. Using cryo-EM and X-ray crystallography, we elucidate structures of sugar phosphates bound to eIF2B and demonstrate that they occupy the ancestral catalytic pocket within the eIF2Bα subunit…
We thank…NanoImaging Services for assistance with cryo-EM data collection and processing.”
Cryo-EM in Mechanism of Action Studies: Epitope Mapping of Monoclonal Antibodies
“...we report the cryo-electron microscopy structures of potent and inhibitory anti-hArg antibodies bound to hArg1 which form distinct macromolecular complexes that are greater than 650 kDa…With local resolutions of 3.5 Å or better we unambiguously mapped epitopes and paratopes for all five antibodies and determined that the antibodies act through orthosteric and allosteric mechanisms. ….Grids were prepared and data were collected at NanoImaging Services (San Diego, CA).”
Absolute Configuration Determination of Pharmaceutical Crystalline Powders by MicroED Via Chiral Salt Formation
“With moderate sample preparation, rapid data processing, and most importantly, unambiguous result interpretation, this [MicroED] approach can serve as a powerful tool in a pharmaceutical scientist's toolbox to determine the absolute configuration of a new API early in drug development, to ensure the quality of drug products, and ultimately, to improve the quality of life of patients.”
NIS Achievements | Our Impact on Drug Discovery and More
150
+
3D structures determined by cryo-EM
76
%
of protein structures solved at 3.5 Å or better resolution
880
+
Virus, Vaccine and VLP projects completed
70
+
Membrane proteins solved
200
+
AAV samples imaged; 3D structure of AAV solved to 1.8 Å
2,500
+
Liposomes & LNPs imaged
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