Single Particle Analysis

NIS Protein Structure
NIS Molecule
NIS Protein Structure

Single particle analysis (SPA) is a group of related computerized image processing techniques used to analyze images from transmission electron microscopy (TEM). These methods were developed to improve and extend the information obtainable from TEM images of samples and allow for high resolution structure determination of proteins, protein complexes and viruses.

We are the global leader in applying SPA techniques to support high resolution, proprietary drug discovery projects.

We are experienced in all stages of single particle analysis needs, from grid preparation and optimization, to high resolution data collection, through processing and refinement of the 3D map. We were the first, and continue to be the only, structural biology CRO with dedicated in-house Titan Krios microscopes, maintaining and expanding state-of-the-art facilities in both San Diego and the Greater Boston area.

We’ve collected data to support over 300 projects, solving over 150 structures, half of which were membrane proteins. Of the structures solved over 76% were determined at resolutions of 3.5 Å or better.

Samples We've Imaged

Antibacterial Targets
Antibody-antigen complexes
DNA & RNA editing enzymes
DNA Binding Proteins
DNA Polymerases & DNA repair
Icosahedral (Adenovirus, AAV)
Ion channels
Macromolecular Complexes
Membrane proteins
Multi-component complexes
Protein degraders
Protein-nucleic acid complexes
Proteins - with and without ligands
Solute Carrier Proteins (SLCs)

Samples We've Characterized

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NIS Mountain Hero
NIS Mountain Hero