Some samples may not tolerate freeze/thaw cycles, or must be stabilized with buffer additives that are incompatible with EM (glycerol, sorbitol, and DMSO). The NIS-East site has a microvolume AKTA Go equipped with Superose 6, Sephadex 200, and desalting columns. These can be used for buffer exchange and removal of soluble aggregates from samples before proceeding to grid preparation. (Note that this service must be requested in advance, and roughly twice as much protein and 50 mL of buffer should be shipped for samples to be run over Superose 6 or Sephadex 200.)
Based on NIS’s accumulated experience with cryo-EM grids, we are innovation leaders in deploying grid variations to help solve some of the most significant sample challenges. For example, we often use continuous support grids to optimize the distribution and orientation of difficult samples; we routinely prepare continuous carbon grids and have developed a reliable method for the preparation of graphene oxide grids, which are ideal for biological macromolecules because of their hydrophilicity and minimal background in cryo-EM imaging.
At NIS we have accumulated decades of experience and established reliable standard operating procedures for both Vitrobot and manual plunging devices. We routinely vitrify soluble proteins, membrane proteins stabilized by detergents, nanodiscs and amphipols, lipid nanoparticles, and formulations such as creams, gels, and emulsions. The Thermo Fisher Scientific Vitrobot and ancillary equipment are available at all of our lab locations.
The Chameleon vitrification method, developed by the founders of NIS, uses a piezo dispensing tip to spray a stream of ~50 pL droplets onto a self-wicking nanowire grid. Physical blotting is eliminated, sample waste and exposure time at the air-water interface are drastically reduced and the use of high-speed cameras allows fine control over a more uniform sample layer thickness.