Getting Started In CryoEM

Working With Us


At NIS, we understand what it takes to establish a successful cryoEM workflow. We have an extensive history of experience helping researchers with their structure projects and a track record of success achieving high resolution results. 


From conception to interpretation and from adoption to long term program sustainability, we can help you implement a cryoEM approach to structure-based drug discovery. 


Getting started in cryoEM usually starts with a proof of concept and every project starts with a free consultation with our experts to discuss the best approach to construct design, the overall goals of the project, timing and budget expectations. 


We have implemented a complete network of resources and range of services designed to meet everyone’s needs. Whether you are sample restricted, time sensitive or budget constrained, we can help you achieve your goals through intelligent project design, implementation and execution. 

Useful Resources

CryoEM Consultation

Request

Krios Reservations

Book

CryoEM Starter eBook

Download

Our Service Offerings

Starter Programs
Microscope Bookings
Partnerships
Fee-For-Service
MicroED
Starter Programs

Single Target Starter Package

The fastest route to sample qualification. This program provides a comprehensive first pass for well-characterized proteins destined for structure determination by Single Particle Analysis. This high value bundle of services includes:

  • Negative stain screening 
  • Vitrification optimization, including chameleon access starting January 2021
  • Overnight data acquisition
  • 2D classification

Designed for high value samples that may be labile, challenging to reproduce, expensive or slow to regenerate or modify, with full workflow access to best-in-class proprietary protocols and leading cryoEM expertise. 


SPA Introductory Program

Explore protein structure for high value targets by cryoEM. Designed for groups with a highly challenging project requiring significant iteration or multiple exploratory projects, our basic Glacios Partnership programs everything needed for a deep dive into protein structure determination by cryoEM:

  • 10 days of reserved Glacios time, including sample prep, screening, overnight data acquisition and 2D classification
  • chameleon access as a standard tool for sample preparation (starting January 2021)
  • Assessment of sample homogeneity by negative stain screening, as needed
  • Up to 10 rounds of conformational and compositional homogeneity analysis via negative stain data acquisition and 2D classification
  • Preferred rates for additional services
  • Onsite training and support as permitted by COVID19-related health considerations/restrictions
  • Remote viewing and regular discussion with a microscopist about screening, image assessment and inference for subsequent optimization and data acquisition
  • Ongoing consultation and project management with a senior scientist of relevant experience to the overarching project goals (SPA)


Glacios Partnership Program

Impact Drug Discovery and Development. Designed for groups with with multiple targets to structurally enable by cryoEM, along with a keen interest to explore the applicability of microED and cryoTEM in broader Drug Discovery and Development applications. 


This program includes:

  • 20 days of reserved Glacios time
  • Single Particle Analysis workflows for sample prep, screening, overnight data acquisition and 2D classification
  • chameleon access as a standard tool for sample preparation (Starting January 2021)
  • Small molecule microED sample preparation and data acquisition
  • Protocol development, sample preparation, screening and data acquisition for protein crystal microED
  • Sample preparation and imaging for biological nanoparticles (liposomes, lipid nanoparticles, viral vectors, and more)
  • Assessment of sample homogeneity by negative stain screening, as needed
  • Up to 10 rounds of conformational and compositional homogeneity analysis via negative stain data acquisition and 2D classification
  • Preferred rates for additional services
  • Onsite training and support as permitted by COVID19-related health considerations/restrictions
  • Remote viewing and regular discussion with a microscopist about screening, image assessment and inference for subsequent optimization and data acquisition
  • Ongoing consultation and project management with a senior scientist of relevant experience to the overarching project goals (SPA)

Pricing for Starter Packages start at $15,000 and represent 40% in savings compared to list prices.


Microscope Bookings

Glacios & Krios Reservations

Whether you need 1 day or 100 each year, we can accommodate. Through our recent collaboration with ThermoFisher Scientific, NIS can add capacity on demand. Our newly expanded facilities and expert staff ensure secure, efficient and responsive access leaving our clients to focus on drug discovery, not instrument acquisition and maintenance.  This access mode is designed for clients with in-house capabilities for sample preparation and data processing requiring regular access to instruments and microscope operators to support maturing programs. Bookings of this type are also popular as a supplement for groups with in-house infrastructure seeking long-term additional capacity or short-term cover.


NIS offers remote realtime viewing of microscope operations, the ability to engage with our operators to agree on acquisition strategy, realtime Cloud-based data streaming and NIST-compliant IT processing for utmost data security and confidentiality.


MicroED Data Acquisition

Intended for groups with in-house expertise in handling small molecule diffraction data, Glacios microscopes located both in San Diego and the Greater Boston Area can be reserved for data collection and preprocessing only. Diffraction images along with necessary instrument settings are returned to the end user for in-house data reduction, phasing, and structure determination. This approach provides clients with ability to meld internal knowhow with access to optimal microED resource, increasing throughput and decreasing costs relative to project-specific proposals.


Partnerships

A consortium approach without consortium logistics.

The Partnership program combines the key attributes of target enablement with firsthand bench experience for industrial researchers. Participants have guaranteed access to best-in-class infrastructure coupled with training nd support from highly experienced staff. These programs provide on-site and remote training for sample preparation through to data acquisition allowing our clients to gain relevant experience while remaining focused on high priority research. Your projects remain your own with no sharing of intellectual property in this or any other access mode. 


Unable to visit our facility on your scheduled day? The dedicated FTE time may be used for included services in the absence of client presence, to provide utmost flexibility in use of time and services.


Partnership days can be combined with any of our other services and instrument access modes to tailor your relationship with NIS in a manner that balances time-to-structure with training commitments.


SPA Introductory Program

Explore protein structure for high value targets by cryoEM. Designed for groups with a highly challenging project requiring significant iteration or multiple exploratory projects, our basic Glacios Partnership programs everything needed for a deep dive into protein structure determination by cryoEM:

  • 10 days of reserved Glacios time, including sample prep, screening, overnight data acquisition and 2D classification
  • chameleon access as a standard tool for sample preparation (starting January 2021)
  • Assessment of sample homogeneity by negative stain screening, as needed
  • Up to 10 rounds of conformational and compositional homogeneity analysis via negative stain data acquisition and 2D classification
  • Preferred rates for additional services
  • Onsite training and support as permitted by COVID19-related health considerations/restrictions
  • Remote viewing and regular discussion with a microscopist about screening, image assessment and inference for subsequent optimization and data acquisition
  • Ongoing consultation and project management with a senior scientist of relevant experience to the overarching project goals (SPA)


Glacios Partnership Program

Impact Drug Discovery and Development. Designed for groups with with multiple targets to structurally enable by cryoEM, along with a keen interest to explore the applicability of microED and cryoTEM in broader Drug Discovery and Development applications. 

This program includes:

  • 20 days of reserved Glacios time
  • Single Particle Analysis workflows for sample prep, screening, overnight data acquisition and 2D classification
  • chameleon access as a standard tool for sample preparation (Starting January 2021)
  • Small molecule microED sample preparation and data acquisition
  • Protocol development, sample preparation, screening and data acquisition for protein crystal microED
  • Sample preparation and imaging for biological nanoparticles (liposomes, lipid nanoparticles, viral vectors, and more)
  • Assessment of sample homogeneity by negative stain screening, as needed
  • Up to 10 rounds of conformational and compositional homogeneity analysis via negative stain data acquisition and 2D classification
  • Preferred rates for additional services
  • Onsite training and support as permitted by COVID19-related health considerations/restrictions
  • Remote viewing and regular discussion with a microscopist about screening, image assessment and inference for subsequent optimization and data acquisition
  • Ongoing consultation and project management with a senior scientist of relevant experience to the overarching project goals (SPA)


Fee-For-Service

No project is too small

Any of our services and instruments can be booked in single unit increments. From negative stain and cryoEM characterization of nanoparticles to Glacios and Titan Krios bookings, your project can be accommodated. All work is performed in close consultation with our scientific team with results available through our convenient online web-based client portal. For data acquisition supporting single particle analysis workflows, remote, real-time web-based viewing of data acquisition is always available. 


Volume based discounts, custom proposals and expedited services are available. 

MicroED

Sample-to-Structure

Our small molecule microED services have been designed in a modular way to ensure maximum flexibility for clients to leverage internal expertise in conjunction with NIS’ capabilities. Phase I efforts will include sample preparation, data acquisition for multiple crystals, data processing, initial phasing, and preliminary refinement. Additional refinement packages are available as Phase II add-ons for clients needing additional support to finalize structures for publication or regulatory filings




Data Acquisition

Intended for groups with in-house expertise in handling small molecule diffraction data, Glacios microscopes located both in San Diego and Greater Boston Area can be reserved for data collection and preprocessing only. Diffraction images along with necessary instrument settings are returned to the end user for in-house data reduction, phasing and structure determination. This approach provides clients with the ability to meld internal knowhow with access to optimal microED resource, increasing throughput and decreasing costs. 


Our Commitment

We are here to support you. At NIS, we offer flexibility, capacity, affordability, and speed to get you through your projects on time and on budget:

  • Flexibility - We recognize that every project is different and needs to be tailored to specific goals. We will work with you to design a project plan with fixed costs, milestones, extensions or whatever else you need to finish successfully.
  • Capacity - Our business model is built around microscope acquisition, which means we will never run out of capacity. We are continuously monitoring the growth of the company and where we need to add resources, infrastructure, or new locations to support our clients. 
  • Affordability - We will work with you to deliver your project within a defined budget and structured payments to minimize risk and reward success.
  • Speed - Your projects should run at your pace and when answers are needed, they are needed quickly. Through our ongoing investments and growth, we are well positioned to align our resources to meet critical timelines for all clients and projects. For complex workflows, we work in close collaboration with our Client's, and when needed our Client's other CROs, to maximize success outcomes at each step and minimize the overall time to project completion.

Get to know our team

Speak with our experts about our services to support your drug discovery efforts.

Contact Us