NanoImaging Services--Services

Proteins and Protein Complex Characterization

Direct visualization of protein and protein complex morphology for vaccines and biologicals are critical to characterization for pharmaceutical and biotechnology process development and formulation functions. NanoImaging Services performs full characterization of nanoscale proteins and protein complexes using advanced cryoTEM (Transmission Electron Microscopy) methods developed specifically to preserve the native biological structure of proteins and protein complexes.

CryoTEM of bacteriophages (Virapur, LLC) showing empty and full capsids


	Morphology Characterization determination of particle shape and size distribution
	Particle Assembly Assessment the only method for direct visual assessment of protein complexes
	3D Structure in silico reconstruction of detailed 3D protein or protein complex model

Applications
	Drug Development: Support for pharmaceutical and biotechnology process development and formulation development and scale-up functions related to chemistry, manufacturing, and control (CMC).
	Regulatory Review: Support for demonstrating that procedures are developed to ensure stability, batch-to-batch uniformity, and overall quality for IND and NDA submissions.
	Validation: Support for demonstration of established protocols and specifications for production process as well as demonstration of bioequivalence when manufacturing protocols are modified or improved.


Services