Cryo-electron Tomography (CryoET)

A 3D volume of the sample can be reconstructed from a series of 2D projection images acquired by incrementally tilting the sample over a range of angles and acquiring a different image for each tilt.   The tilted set of projection images can then be mathematically combined to reconstruct a 3D volume of the sample, essentially using tomographic methods (analogous to those used for CT scanning and other medical imaging procedures). The 3D volume can be examined from different angles and individual slices in different orientations can be extracted to provide a more complete understanding of the particle morphology and resolve any ambiguities of the 2D projection images.

These analyses help answer questions like:

  • 3D Shape
    What is the shape of particles in 3D relative to their 2D projection images?
  • Drug Delivery Vehicle Structure
    What is the distribution of proteins on the surface of drug delivery vehicles?
  • Drug Delivery vehicle cargo
    How are encapsidated drugs arranged inside the drug delivery vehicle?
  • Lamellarity Determination
    How many continuous lipid layers do (multilamellar) liposomes contain?
  • Interactions
    Can interactions between sample components be confirmed (e.g. vaccine particles and adjuvants)?