Characterization of Liposomes in Solution
The Challenge: Characterization of Liposomes in Solution
A company engaged in the development of novel delivery methods for small molecule therapeutics needs accurate determinations of liposomal morphology (size distribution and shape), and assembly (lamellarity) of lipid particles. However, common methods of size determination such as Dynamic Light Scattering (DLS) are not direct measures of size, are hampered by heterogeneous samples, and provide no information regarding shape or lamellarity. Direct visualization of nanoscale liposomes by freeze fracture TEM is prone to distortion and artifacts from sample preparation and does not provide the critical structural details (i.e. lamellarity).